# Run the pipeline ## Initialize your project 1. Open a terminal 2. Activate your environment `conda activate ipasuite` 3. Generate your project folder `ipasuite init [project]` A project folder will be created with all the necessary files for workflow execution You can access this folder by a file browser or doing `cd [name of your project]` ## Configure your project 4. Configure your pipeline using the [configurator](configuration) `cd [name of your project]; ipasuite config` 5. Fill up your experiments in `samples.tsv` specifing where to find your raw data ([See configuration](configuration)) and QuShape projects if you already treated some experiments ## Run pipeline 6. Run `ipasuite qushape` it will run the first part of the workflow and open QuShape for each file to treat. You must follow every step of QuShape and save the QuShape file. You can follow the [QuShape Tutorial of the Weeks lab](QuShape_Tutorial.pdf) or the version of the Sargueil lab. 7. Run `ipasuite run` `ipasuite` will generate structure models in results/5.3-structure and results/5.4-varna treated with ipanemap as set up in your configuration file ## In case of trouble : Two commands can help debugging : ```bash ipasuite check ``` Will check : - If some input files are missing - If all necessary samples are available to run IPANEMAP and the footprint analysis - If samples are duplicated - If some raw files are identical ```bash ipasuite log ``` Will show pipeline scripts log ## Clean file at any point, you can clean pipeline files : ```bash ipasuite clean ``` It will remove by default all files, figures and logs downstream qushape treatement. You can choose from which step to remove file using `--from-step` To only clean log files: ```bash ipasuite log --clean ```